Countercurrent Chromatography of an Anti-trypanosomal Factor from Pseudomonas Fluorescens

Abstract

Countercurrent chromatography (CCC) employing the Ito multilayer coiled column was an effective procedure for the purification of an anti-trypanosomal factor (ATF-II) from Pseudomonas fluorescens. Two types of anti-trypanosomal activity were observed, i.e., lysis, and paralysis and killing of the parasites without lysis. High performance liquid chromatography (HPLC) was very useful for the differentiation and further purification of these fractions, and bioassays performed with Trypanosoma equiperdum, indicated the characteristic pathologic changes produced by these components. The lipopolysaccharide content of the purified fractions was markedly reduced and indicated an additional advantage of CCC in future studies with experimental infections with T. cruzi.