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Archives of Andrology
Journal of Reproductive Systems
Volume 52, 2006 - Issue 5
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Assisted Reproduction

QUANTITATIVE ANALYSIS OF CCR5 CHEMOKINE RECEPTOR AND CYTOCHROME P450 AROMATASE TRANSCRIPTS IN SWIM-UP SPERMATOZOA ISOLATED FROM FERTILE AND INFERTILE MEN

P. Jedrzejczak Department of Infertility and Reproductive Endocrinology, Poznań University of Medical Sciences, Poznań, Poland
,
R. Januchowski Department of Biochemistry and Molecular Biology, Poznań University of Medical Sciences, Poznań, Poland
,
G. Taszarek-Hauke Department of Infertility and Reproductive Endocrinology, Poznań University of Medical Sciences, Poznań, Poland
,
R. Laddach Department of Biochemistry and Molecular Biology, Poznań University of Medical Sciences, Poznań, Poland
,
L. Pawelczyk Department of Infertility and Reproductive Endocrinology, Poznań University of Medical Sciences, Poznań, Poland
&
P. P. Jagodzinski Department of Biochemistry and Molecular Biology, Poznań University of Medical Sciences, Poznań, PolandCorrespondence[email protected]
Pages 335-341 | Published online: 09 Jul 2009
 
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Abstract

We determined the CCR5 chemokine receptor and cytochrome P450 aromatase (P450arom) transcript copies number in swim-up sperm isolated from fertile and infertile men. The ejaculates were purified by centrifugation through discontinuous Percoll density gradient and swim-up techniques. RNA was isolated from sperm, treated with DNase I and reverse-transcribed into cDNA. Quantitative analysis of CCR5 and P450arom cDNA were performed by real-time quantitative (RQ-PCR) SYBR Green I analysis. There was a higher content of CCR5 and P450arom transcripts copy number in swim-up sperm of fertile than from infertile donors. The decrease in CCR5 and P450arom transcripts in swim-up sperm may be associated with male infertility.

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