Microbial Diversity in Uranium Deposits from Jaduguda and Bagjata Uranium Mines, India as Revealed by Clone Library and Denaturing Gradient Gel Electrophoresis Analyses

Abstract

Microbial communities within subsurface uranium (U) deposits were explored to understand the nature of community composition and their potential role in biogeochemical cycle and bioremediation. Geochemical analysis revealed that the U ores were mainly hosted on metamorphosed chlorite-biotite schists, containing varied organic carbon and elevated level of several heavy metals (U, Cu, Cr, Zn, etc.). Microbial diversity as explored by 16S rRNA gene clone library and DGGE analyses revealed predominance of Proteobacteria, Acidobacteria, Bacteroidetes along with Firmicutes and candidate division OP9 within the domain Bacteria and Euryarchaeota within the domain Archaea. Among the physiochemical parameters, level of organic carbon showed considerable impact on influencing community diversity and composition. Samples from Jaduguda with high organic carbon showed abundance of bacteria known for metabolizing different carbon compounds and affiliated to unclassified uncultured members of Chitnophagaceae (Bacteroidetes), Gp4 of Acidobacteria and unclassified β-Proteobacteria and halophilic, nitrate-reducing γ-Proteobacteria. A relatively diverse assemblage of species capable of autotrophic/heterotrophic N₂ fixation, CH₄ utilization, H₂(0)/Fe(II)/Mn(II)/S(0) oxidation, NO₃/Fe(III) reduction, U and other metal precipitation/mineralization and affiliated to families Rhizobiaceae, Bradyrhizobiaceae, Caulobacteraceae, Comamonadaceae and genera Acinetobacter, Marinobacter and Alcanivorax constituted the Bagjata samples. Distribution and interrelations among abundance of various bacterial groups detected in other U mines/radioactive waste sites were compared with our data. Overall, the study reported distinct compositions of indigenous microbial communities among the samples from two mines and provided a better insight in geomicrobiology of U deposits.

Keywords:

• 16S rRNA gene
• ARDRA
• microbial diversity
• PCR-DGGE
• uranium ore deposit

Acknowledgments

Critical comments on the manuscript by Dr. Sufia K. Kazy, Department of Biotechnology, National Institute of Technology, Durgapur are acknowledged. The authors thank to Prof Biswajit Mishra and Dr. Dewashish Upadhaya, Department of Geology and Geophysics, Indian Institute of Technology Kharagpur for their generous help in petrological and EPMA analysis. Authors are thankful to Uranium Corporation of India Ltd., Jaduguda for their kind support during sampling in mines.

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Funding

This study was supported by grants from BRNS, Department of Atomic Energy, Government of India. Ekramul Islam and Dhiarj Paul acknowledge financial assistance received from Council of Scientific and Industrial Research, India and University Grants Commission (UGC), India.