Bioimmobilization of Heavy Metals in Acidic Copper Mine Tailings Soil

ABSTRACT

Heavy metal contamination of mine tailings is one of the most serious environmental challenges facing the mining industry worldwide. Conventional technologies used for the treatment of such tailing soils are expensive both in terms of operation and capital costs as well as being not so effective. In the present study, an indigenous calcifying urease producing bacterial strain was isolated from copper mine tailing soil and used for bioimmobilization of copper, lead and cadmium in contaminated mine tailing soils. Phylogenetic analysis of the 16S rRNA gene sequence identified it as Bacillus firmus. The efficiency of metal bioimmobilization was based on microbially induced calcite precipitation (MICP). A five-stage sequential soil extraction procedure was carried out to obtain distribution patterns for the different metals. The mobility of the toxic metals was found to be significantly reduced in the exchangeable fraction with their concentrations markedly increasing in the carbonated fraction after bioremediation. Scanning electron microscopy showed the precipitation of calcite in association with the bacterial cells. Calcium carbonate minerals such as calcite, gwihabaite and aragonite were identified in the bioremediated tailings soils using X-ray diffraction (XRD). It is concluded that MICP holds considerable promise for the remediation of mine tailing soils by efficient immobilization of toxic metals such as copper, lead and cadmium.

KEYWORDS:

• Bacillus firmus
• bioremediation
• cadmium
• lead
• mine tailings
• urease

Funding

This work was supported by the National Natural Science Foundation of China (U1120302, U1403181, 21177127, 31360027 and 41203088), the West Light Foundation of Chinese Academy of Science (XBBS201304) and the China Postdoctoral Science Foundation Funded Project (131622). This project was also supported by the Research Center, College of Science, King Saud University, Riyadh 11451, KSA.