An Efficient Strategy Based on Macroporous Resins and Semi-Preparative High Performance Liquid Chromatography for Rapid Separation of Five Flavonoids Components From Flaveria Bidentis(L.) Kuntze

Abstract

In this study, a simple, rapid, and efficient purification method of five major flavonoids from crude Flaveria bidentis extracts was established. Five flavonoids components were initially obtained by ethanol-water extraction of Flaveria bidentis (L.) Kuntze, followed by using D4020 resin-based column chromatography and semi-preparative high performance liquid chromatography. 9.3 mg hyperoside, 6.5 mg patuletin-3-O-glucoside, 1.7 mg isorhamnetin 3-sulphate, 8.4 mg astragalin, and 4.9 mg 6-methoxykaempferol-3-O-galactoside at high purity of over 94% were obtained from 580 mg Flaveria bidentis extracts. This method is more efficient than HSCCC comparing with the results of these two methods regarding analysts’ purity, recovery, and running time.

Keywords:

• flavonoid
• macroporous resins
• purification
• semi-preparative high performance liquid chromatography

ACKNOWLEDGEMENTS

Financial support by Special Fund for Agro-scientific Research in the Public Interest (project 200803022 and 201103027), Program for New Century Excellent Talents in University (NCET-11-0563) and the National Natural Science Foundation of China (Grant No. 21075007) are gratefully acknowledged.

Notes

Note: the flow rate varied while the sample loading was kept at 100 mg.

Note: the sample loading varied while the flow rate was kept at 12 mL/min.