Preparative Separation and Purification of Three Flavonoids from the Anti-Inflammatory Effective Fraction of Smilax china L. by High-Speed Counter-Current Chromatography

Abstract

Flavonoids are the main chemical constituents of Smilax china L. and thought to be responsible for the anti-inflammatory activity of Smilax china L. In this study, a suitable HSCCC method was developed to separate the three main flavonoids in a one-separation from the anti-chronic pelvic inflammation disease (CPID) effective fraction of Smilax china L. with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:10:1:10, v/v/v/v). 42 milligrams of astilbin, 10 mg neoisoastilbin, and 6 mg quercetin-3-O-α-L-rhamnoside were separated from 200 mg of the anti-CPID effective fraction of Smilax china L. with purity of 98.3%, 98.7%, 98.5%, respectively, by HPLC analysis. Orthogonal test L₉ (3⁴) was also applied to investigate the optimum extracting conditions of the three flavonoids. HSCCC was successfully used for the isolation and purification of the three flavonoids from the anti-CPID effective fraction of Smilax china L. and the neoisoastilbin was first separated from Smilax china L.

Keywords:

• astilbin
• high-speed counter-current chromatography
• neoisoastilbin
• quercetin-3-O-α-L-rhamnoside
• Smilax china L

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