Single step recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction

ABSTRACT

Recovery of lipase from Penicillium cyclopium by aqueous two-phase extraction was studied with maximal possible crude enzyme loads. In polyethylene glycol/dextran and polyethylene glycol/salt systems the influences of molecular weight and concentration of polyethylene glycol, phase-forming salt and phase volume ratio were evaluated. Lipase partition coefficient 9 followed by the top phase yield 95.7% and purification factor 3.4 were achieved in 15% (w w⁻¹) polyethylene glycol 4000/15% (w w⁻¹) KH₂PO₄/70% (w w⁻¹) crude enzyme. Efficient single-step recovery of lipase followed by partial enzyme purification indicated possible integration of production and primary bioseparation step by aqueous two-phase extraction. By varying phase volume ratio, the concentration of phosphate was reduced without decrease in lipase recovery.

KEYWORDS:

• Aqueous two-phase extraction
• bioseparation
• lipase
• partitioning
• recovery

Nomenclature

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Additional information

Funding

Financial support from the Ministry of Education, Science and Technological Development of the Republic of Serbia (Grant No. III 46010) is gratefully acknowledged.