Identification of bioactive compounds with GC–Q-TOF–MS in the extracts from Clinacanthus nutans using subcritical carbon dioxide extraction

Abstract

Subcritical carbon dioxide Soxhlet extraction of biologically active compounds from Clincanthus nutans was investigated by full factorial design to identify and optimize the factors (particle size and co-solvent) affecting extract yield, antioxidant activity, total phenolic content, total flavonoid content, and α-glucosidase inhibitory activity. An average of 3.103% yield, 98.90% antioxidant activity, 49.40 mg/g (GAE) TPC, 43.76 mg/g (RE), and 88.58% AGI activity can be achieved using the optimum levels of independent variables. The GC-Q-TOF MS identification of optimized extract shown that different classes of phytoconstituents were successfully separated by CO₂-Soxhlet to produce potential antioxidant and α-glucosidase inhibitory activity.

Keywords:

• α-glucosidase inhibitory activity
• antioxidant
• Clinacanthus nutans
• subcritical carbon dioxide extraction

Funding

The authors would like to acknowledge Malaysian International Scholarship, Ministry of Higher Education, Malaysia. The authors extend their appreciation to the International Scientific Partnership Program ISPP at King Saud University, Riyadh, Saudi Arabia, for funding this research work through ISPP# 0026.

Additional information

Funding

The authors would like to acknowledge Malaysian International Scholarship, Ministry of Higher Education, Malaysia. The authors extend their appreciation to the International Scientific Partnership Program ISPP at King Saud University, Riyadh, Saudi Arabia, for funding this research work through ISPP# 0026.