Effect of operating conditions on catechin extraction from betel nuts using supercritical CO₂-methanol extraction

ABSTRACT

In this work, the effect of extraction parameters on catechin extraction from betel nuts was studied. The supercritical carbon dioxide extraction parameters were pressure, temperature, and solvent flow rate while using average particle size of 177.5 μm and 5% (v/v) methanol as modifiers. Detection and quantification of catechin were achieved using HPLC analysis. The highest amount of extract and catechin concentration were 34.00 mg extract/g sample and 565.38 ppm of catechin obtained at 30 MPa, 70°C, and 4 mL/min. The results given by optimization tools suggest the same operating conditions with less than 3% difference.

KEYWORDS:

• Areca catechu
• betel nut
• catechin
• supercritical fluid extraction
• optimization

Funding

This research is funded by Malaysian government under Research University Grant (GUP) (Vote no: Q.J130000.2544.04H05), Ministry of Higher Education Malaysia with collaboration of Centre of Lipids Engineering and Applied Research (CLEAR), Universiti Teknologi Malaysia (UTM), Malaysia.

Additional information

Funding

This research is funded by Malaysian government under Research University Grant (GUP) (Vote no: Q.J130000.2544.04H05), Ministry of Higher Education Malaysia with collaboration of Centre of Lipids Engineering and Applied Research (CLEAR), Universiti Teknologi Malaysia (UTM), Malaysia.