Chemometric analysis of selective polyphenolic groups in Asparagus racemosus (Shatavar) root extracts by traditional and supercritical fluid (CO₂) based extractions

ABSTRACT

Asparagus racemosus root extracts were prepared by supercritical fluid (CO₂), soxhlet, and maceration-based methods also with various pretreatments. Thereafter, these root extracts were analyzed by High-Performance Liquid Chromatography, along with the chemometric study of the disparate phenolic groups. Among these, supercritical fluid (CO₂) based extract has a larger number of polar compounds and the antioxidant activity (98.54 ± 0.22 µM Trolox equivalent mg⁻¹). It also has the best cell viability (94.37 ± 1.12%) and insulin release (0.82 ± 1.12 ng mL⁻¹) on β-pancreatic RINm-5F cells whereas, the best extractive yield (75.80 ± 3.44% w/w) was observed for pretreated aqueous soxhlet-based extract.

KEYWORDS:

• Asparagus racemosus
• polyphenols
• PCA
• supercritical fluid extraction
• sonication

Acknowledgments

The author thanks the university grant commission, ministry of human resource and development, India for providing the Basic Scientific Research grant fellowship to carry out the research work.

Supplementary material

Supplemental data for this article can be accessed here.