Enzymatic synthesis of chiral alcohols using ketoreductases

ABSTRACT

Ketoreductases(KREDs), which play an important role in the preparation of chiral alcohol drug intermediates, are promising biocatalysts that can lead chiral alcohol preparation to become a greener and more sustainable process. Two decades ago, KREDs were widely researched from protein structure to catalytic mechanism, and many researchers worked on catalytic properties research. And from decades ago, with protein engineering development, KREDs become useful in the chiral alcohol production process. This review has summarized KREDs from protein structure to applications, from the perspective of biocatalysis, providing a detailed introduction to different KRED structures and many applications, as well as the enzyme library construction and bioreactor applications.

Graphical abstract

KEYWORDS:

• Biocatalysis
• chiral alcohols
• ketoreductases
• pharmaceutica
• protein

Acknowledgments

This work was supported by the National Key Research and Development Program of China (2023YFA0914500), the National Natural Science Foundation of China (No. 22378096, 22308083, 22178083 and 22078081), the Natural Science Foundation of Hebei Province (C2019208174 and B2022202014), the S&T program of Hebei (21372805D, 21372804D), the Educational Commission of Hebei Province (JZX2023012).

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by the National Key Research and Development Program of China [2023YFA0914500]; National Natural Science Foundation of China [No. 22378096, 22308083, 22178083 and 22078081]; Natural Science Foundation of Hebei Province [C2019208174 and B2022202014]; the educational commission of hebei [JZX2023012]; S&T program of Hebei [21372805D, 21372804D].