Functional, electrophysiological recoveries of rats with sciatic nerve lesions following transplantation of elongated DRG cells

ABSTRACT

Objectives: Functional data are essential when confirming the efficacy of elongated dorsal root ganglia (DRG) cells as a substitute for autografting. We present the quantitative functional motor, electrophysiological findings of engineered DRG recipients for the first time.

Methods: Elongated DRG neurons and autografts were transplanted to bridge 1-cm sciatic nerve lesions of Sprague Dawley (SD) rats. Motor recoveries of elongated DRG recipients (n=9), autograft recipients (n=9), unrepaired rats (n=9) and intact rats (n=6) were investigated using the angle board challenge test following 16 weeks of recovery. Electrophysiology studies were conducted to assess the functional recovery at 16 weeks. In addition, elongated DRGs were subjected to histology assessments.

Results: At threshold levels (35° angle) of the angle board challenge test, the autograft recipients’, DRG recipients’ and unrepaired group’s performances were equal to each other and were less than the intact group (p<0.05). However, during the subthreshold (30°) angle board challenge test, the elongated DRG recipients’ performance was similar to both the intact group and the autograft nerve recipients, and was better (p<0.05) than the unrepaired group. The autograft recipients’ performance was similar to the unrepaired group and was significantly different (p<0.05) compared with the performance of the intact group. During electrophysiological testing, the rats with transplanted engineered DRG constructs had intact signal transmission when recorded over the lesion, while the unrepaired rats did not. It was observed that elongated DRG neurons closely resembled an autograft during histological assessments.

Conclusion: Performances of autograft and elongated DRG construct recipients were similar. Elongated DRG neurons should be further investigated as a substitute for autografting.

Keywords:

• Peripheral nerve injury
• DRG; nerve elongation
• functional recovery

Abbreviations:

• DRG:dorsal root ganglia
• SD: Sprague Dawley

Acknowledgement

Authors would like to thank Mr. Bryan Moss for the illustration (Fig. 1) and Mr. Glen Cryer for helping us prepare the manuscript.