Association of promoter polymorphism -857C/T (rs1799724) in tumor necrosis factor gene with intracerebral hemorrhage in Korean males

Abstract

Background and purpose: Tumor necrosis factor (TNF) has been shown to be involved in the pathogenesis of hemorrhagic stroke, having deleterious effects on cerebral arteries by promoting inflammation and apoptosis in vascular and immune cells. In this study, we investigated genetic association between TNF gene and intracerebral hemorrhage (ICH) in a Korean population. Methods: Single nucleotide polymorphisms (SNPs) of TNF gene [−857C/T (rs1799724) and -308G/A (rs1800629)] were selected and genotyped using direct sequencing in 144 ICH patients and 455 control subjects. Genotype distribution and allele frequency were compared between cases and controls using logistic regression.

Results: −857C/T was significantly associated with ICH in log-additive [odds ratio (OR) = 1.60, 95% confidence interval (CI) = 1.14–2.24, p = 0.0081], and recessive models (OR = 3.25, 95% CI = 1.28–8.27, p = 0.016). The frequency of the −857TT genotype increased in ICH patients. Allele frequency analysis also showed that the −857T allele was associated with an increased risk of ICH (OR = 1.62, 95% CI = 1.15–2.30, p = 0.006). In the analysis according to the gender, we found that the association of −857C/T was gender–different. The −857C/T was significantly associated with ICH only in males (OR = 1.99, 95% CI = 1.24–3.19, p = 0.0043 in males; OR = 1.30, 95% CI = 0.76–2.22, p = 0.34 in females).

Conclusion: These results suggest that promoter polymorphism of TNF gene, −857C/T, may be involved in the susceptibility of ICH in males.

Keywords:

• Tumor necrosis factor
• intracerebral hemorrhage
• single nucleotide polymorphism

Notes on contributors

Hae Jeong Park , PhD, and Su Kang Kim , PhD, are assistant professors in the Department of Pharmacology (Kohwang Medical Research Institute) at Kyung Hee University.

Hyun-Kyung Park is an associate professor in the Department of Emergency Medicine at Kyung Hee University. She takes interests in pathological mechanism and clinical study of stroke.

Joo-Ho Chung is a professor in the Department of Pharmacology (Kohwang Medical Research Institute) at Kyung Hee University. Their research interest is identifying genetic markers of neurological and psychiatric diseases via case-control association study and functional study on SNPs.