Neuroprotective ability of TMV coat protein on rat PC-12 cells and it’s in silico study with LRRK2 receptor

ABSTRACT

Objective: Present study was to observe the neuroprotective ability of TMV coat protein by observing both in vitro studies on Rat PC-12 cells and in silico studies with LRRK2 receptor by molecular docking.

Methods: TMV coat protein was extracted out from the stem of Nicotiana tabacum and was purified and identified by MALDI-TOF/MS/MS analysis. We confer antioxidant activity of TMV coat protein by enzyme activity like superoxide dismutase (SOD), catalase (CAT) and glutathione-s-transferase (GST) and nonenzyme content by glutathione (GSH) and malondialdehyde (MDA) content. Neuroprotective ability of TMV coat protein was observed by determining the enzyme activity and nonenzyme content in treated cells that were exposed to neurotoxic shock. In silico studies were done in order to observe the molecular docking studies against LRRK2 receptor.

Results: Antioxidant content was found to be high in TMV coat protein and in treated Rat PC-12 cells as well. Enzyme activity and nonenzyme content were determined and their levels were found to be in increasing level with respect to the volume of 0.2 mg/ml of TMV coat protein. In silico studies revealed the binding efficacy of TMV coat protein with LRRK2 receptor by observing the molecular docking using automated servers.

Conclusion: From the present study, it was found that TMV coat protein can be utilized as neuroprotective agent and inhibitor of LRRK2 receptor.

KEYWORDS:

• Tobacco Mosaic Virus
• Superoxide dismutase
• Catalase activity
• Glutathione s transferase
• Lipid peroxidation
• Neuroprotection

Acknowledgments

We authors would like to acknowledge our Dr. Nupur Sinha Srivastava, Assistant Professor and Dr. Chandardeep Tandon, Director, Amity Institute of Biotechnology, Amity University, Noida, India for their valuable guidance and support.

Disclosure statement

The author(s) declare(s) that there is no conflict of interest regarding the publication of this paper.

Additional information

Notes on contributors

Yash Sharma

Mr. Yash Sharma is a Ph.D Research Scholar in Amity Institute of Biotechnology, Amity University Uttar Pradesh, working on polyphenolic flavonoid analysis of different medicinal herbs and it’s nanoformulation for Drug delivery.

Nidhi Srivastava

Dr. Nidhi Srivastava has specialization in cellular and molecular mechanism of wound healing. Currently, she is working on biomarkers of angiogenesis and immunological disorders.

Kumud Bala

Dr. Kumud Bala, corresponding author, has specialization in Immunology and cancer, where she has worked on cytokine profiling, immunohistochemistry, chromosome deformation, site directed mutagenesis. She is currently working on herbal remedies for neurodegenerative agents and its drug delivery system in in vitro and in vivo condition.