Cranberry Juice Constituents Impair Lymphoma Growth and Augment the Generation of Antilymphoma Antibodies in Syngeneic Mice

Abstract

In addition to its nutritional value, cranberry juice has been effective in treating urinary tract infections. Various reports have also demonstrated its potential for inhibiting in vitro growth of transformed cell lines. Here we show that a fraction [nondialyzable material (NDM) of a molecular weight range 12,000–30,000 (NDM 12-30K)] derived from cranberry juice impairs in vitro growth and invasion through extracellular matrix of Rev-2-T-6 murine lymphoma cells. Furthermore, intraperitoneal injection of this fraction at nontoxic doses both inhibits the growth of Rev-2-T-6 tumors in vivo and enhances the generation of antilymphoma antibodies. These findings demonstrate the in vivo efficacy of cranberry components against malignant lymphoma in immune competent hosts.

ACKNOWLEDGMENTS

The authors thank I. Ofek and the Israel Cranberry team for stimulating discussions.

Notes

^a Rev-2-T-6 cells (5 × 10⁶/mouse) were inoculated intraperitoneally into mature (6 wk old) female Balb/C mice. Abbreviations are as follows: NDM, nondialyzable material; NDM (12-30K), NDM molecular weight range 12,000–30,000.

^b 2 mg of NDM (12-30K) were inoculated intraperitoneally 1 day after Rev-2-T-6 inoculation. This was followed by inoculation of 1 mg of NDM (12-30K) every other day for 14 days.

^c 4 mg of NDM (12-30K) were inoculated intraperitoneally 1 day after Rev-2-T-6 inoculation. This was followed by inoculation of 2 mg of NDM (12-30K) every other day for 14 days.