Effect of Coffee on Chemical Hepatocarcinogenesis in Rats

Abstract

The aim of this study was to investigate the effect of daily coffee ingestion on hepatocarcinogenesis in rats submitted to the resistant hepatocyte (RH) model. During lactation, the dams were fed a control or a coffee-supplemented diet. After weaning, male pups followed the same dietary protocol and were submitted to the RH model. The animals were sacrificed at 110 days of life. Removal of the medial and left lateral lobes was used as mitogenic stimulus, and the liver regeneration was estimated. Morphometric analyses of preneoplastic lesions were carried out on liver histological sections submitted to the histochemical procedure of the glucose-6-phosphatase activity. The γ-glutamyltranspeptidase (GGT) activity was analyzed in the homogenate of regenerated livers. Body weight, mass liver regeneration, and hepatic cell architecture were not affected by coffee ingestion. In the group of animals fed the coffee-supplemented diet, the number of persistent and remodeling nodules was reduced (85.5% and 70.5%, respectively). The hepatic area occupied by the persistent nodules was also reduced (92%). There was a reduction of 7.7% in the GGT activity in the group fed the coffee-supplemented diet, although not statistically significant. The results indicate that coffee modulates chemical hepatocarcinogenesis in rats.

ACKNOWLEDGMENTS

This study was supported by Capes (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), and Fapemig (Fundação de Amparo à Pesquisa do Estado de Minas Gerais). We thank André R. Massensini for the helping with the manuscript writing.