Quercetin Inhibits Diethylnitrosamine-Induced Hepatic Preneoplastic Lesions in Rats

Abstract

Quercetin is an antioxidant flavonoid, found ubiquitously in nature and extensively used in herbal medicines and food additives. This study aimed to investigate the effect of quercetin on diethylnitrosamine-induced preneoplastic lesions, using the medium-term rat liver bioassay. The γ-benzene hexachloride was used as promoter at the doses of 0.1, 1.0, and 10.0 mg/kg against a single dose of diethylnitrosamine (200 mg/kg) in male Sprague-Dawley rats. All the rats were subjected to 70% partial hepatectomy at Week 4. The protective effect of quercetin (5 and 25 mg/kg) was examined against the highest dose of γ-benzene hexachloride (10 mg/kg). A significant increase in the number as well as the mean area of glutathione S-transferase placental form (GST-P) positive foci, p53 positive hepatocytes, and the percentage of apoptotic cells were observed in the diethylnitrosamine-treated group. In the present investigation, both doses of QC (5 and 25 mg/kg) led to a significant decrease in the number as well as the mean area of GST-P positive foci, TUNEL positive apoptotic cells, p53 positive hepatocytes, and restoration of cellular morphology. These results clearly indicate that quercetin inhibits diethylnitrosamine-induced hepatic preneoplastic lesions in medium-term rat liver bioassay.

ACKNOWLEDGMENTS

The financial assistance received from the National Institute of Pharmaceutical Education and Research (NIPER), Mohali is acknowledged, as is the financial assistance awarded to Mr. D. N. Tripathi by CSIR letter no. 111172/2K8/1. We are grateful to the anonymous reviewers for critically evaluating our results and the valuable comments to further increase the quality of the manuscript. We also place our gratitude to Professor P. Tiwari, Department of Pharmacy Practice, NIPER, SAS Nagar, Punjab, India for his expert language editing.