Molecular Mechanism of Antiproliferation Potential of Acacia Honey on NCI-H460 Cell Line

Abstract

Lung cancer is one of the leading causes of death worldwide. We investigated the molecular mechanism of antiproliferation potential of Acacia honey on NCI-H460 cells by cell cycle, viability, cytokines, calcium ion and gene expression analysis. Acacia honey inhibited cells proliferation, arrested G0/G1 phase, stimulated cytokines, calcium ion release as well as suppressed p53 and Bcl-2 expression in a dose-dependent manner. We proposed that the molecular mechanism of the antiproliferation potential of Acacia honey on NCI-H460 cell line is due to cell cycle arrest, stimulation of cytokines and calcium ion as well as downregulation of Bcl-2 and p53 genes.

ACKNOWLEDGMENTS

We appreciate the consistent laboratory effort of Mudassir Azhar, Abbas Gulam, Izhar Mirani, Faheema Hafeez Siddiqi, Sabira Naqvi, Tamkeen, Rohina and Nadia from Pharmacology Research Laboratory, H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan. This research work was fully funded and supported by the International Centre of chemical and Biological Sciences, ICCBS, University of Karachi, Karachi, Pakistan.