Dietary Polyamine Intake and Polyamines Measured in Urine

Abstract

Dietary polyamines have recently been associated with increased risk of pre-malignant colorectal lesions. Because polyamines are synthesized in cells and taken up from dietary sources, development of a biomarker of exposure is challenging. Excess polyamines are primarily excreted in the urine. This pilot study seeks to identify dietary correlates of excreted urinary polyamines as putative biomarkers of exposure. Dietary polyamines/other nutrients were estimated from a food frequency questionnaire (FFQ) and correlated with urinary levels of acetylated polyamines in 36 men using 24-h urine samples. Polyamines, abundant in cheese and citrus, were highly positively correlated with urinary N⁸-acetylspermidine (correlation coefficient; r = 0.37, P = 0.03), but this correlation was attenuated after adjustment for total energy intake (r = 0.07, P = 0.68). Dietary energy intake itself was positively correlated with urinary total acetylated polyamine output (r = .40, P = 0.02). In energy-adjusted analyses, folic acid and folate from food were associated with urinary N¹,N¹²-diacetylspermine (r = 0.34, P = 0.05 and r = −0.39, P = 0.02, respectively). Red meat negatively correlated with total urinary acetylated polyamines (r = −0.42, P = 0.01). Our findings suggest that energy, folate, folic acid, saturated fat, and red meat intake, as opposed to FFQ-estimated dietary polyamines, are correlated with urinary polyamines.

ACKNOWLEDGMENTS

We would like to acknowledge Dr. Tetsuya Kosaka and the Alfresa Pharma Corporation research group for conducting the N¹,N¹²-diacetylspermine analysis. We would also like to thank David Stringer for analyzing the parent and monoacetyl polyamines in our urine samples.

SUPPLEMENTAL MATERIAL

Supplemental data for this article can be accessed on the publisher's web site, www.tandfonline.com/hnuc.