Pro-Apoptotic Activities of Polyphenolics From Açai (Euterpe oleracea Martius) in Human SW-480 Colon Cancer Cells

Abstract

This study aimed to evaluate the cell growth inhibition activity of açai (Euterpe oleracea Mart.) polyphenolic extract against colon cancer HT-29 and SW-480 cells and the nonmalignant CCD-18Co colon fibroblast cells. Results showed that açai polyphenolic extract (5–20 mg/L) inhibited preferentially the growth of SW-480 cells with no toxicity in CCD-18Co cells, and this was accompanied by reduction of H₂O₂-induced reactive oxygen species (ROS) generation. The mechanisms involved in SW-480 cell growth-inhibition by açai polyphenolic extract included the downregulation of NF-κB proinflammatory transcription factor and the nuclear factor-kappa B targets intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). Furthermore, prooncogenic specificity proteins (Sp) were downregulated as well as Sp-targets Bcl-2, vascular endothelial growth factor, and survivin. This was accompanied by activation of mitochondrial proapoptotic pathway involving increase of cytochrome c, cleavage of caspase-3, and decrease of PARP-1. Results strongly suggest that açai polyphenolic extract has antiinflammatory and cytotoxic activities in colon cancer cells and can be effective as natural colon cancer chemopreventive agents.

ACKNOWLEDGMENTS

We thank Ms. Andrea Roque at Texas A&M University for her technical support with Western blotting and Ms. Alexandria Bass at Texas A&M University for her support with manuscript editing.

Additional information

Funding

We thank the National Counsel of Technological and Scientific Development (CNPq) from Brazil with funding ref. number (200548/2011-5) for partially financially supporting this project.