Induction of Apoptosis in HeLa by Corn Small RNAs

Abstract

Insights in RNA biology have opened up a plethora of opportunities to explore the small regulatory RNAs from various natural and artificial sources. These small RNAs have been suggested to play a role too in tumor progression by either as oncogenic or tumor suppressor small RNAs. In this study, authors have attempted to evaluate the therapeutic potential of small RNAs fractionated from corn (Zea mays) upon growth and survival of HeLa. Here, authors have employed standard cellular-based approaches including microscopy, spectroscopy, and flow cytometry-based staining assays. Our data indicate that corn small RNAs fraction can appreciably decrease HeLa cell proliferation and survival, which is supported by a number of complementary assays such as Trypan blue dye exclusion, MTT, propidium iodide, and Annexin V/PI apoptotic cell death. Taken together, present finding suggests that corn small RNAs fraction may display up to 70% reduction in HeLa cell viability. Furthermore, these data indicate that around 40–50% of HeLa cells become apoptotic due to exogenous use of corn small. Overall, this finding proposes that possibility of cross-kingdom anticancer use of small RNAs from corn and present data need to be explored in depth.

Acknowledgments

The authors acknowledge Central Research Facility, DPU, Pune for flow cytometer and fluorescent microscopy experimentation facilities.

Disclosure Statement

The authors have no conflict of interest to declare.

Additional information

Funding

The authors acknowledge financial support from Dr D. Y Patil, Vidyapeeth, Pune, India as an intra-mural grant (DPU/05/01/2016) and DST-SERB, Government of India, New Delhi, India (SERB/LS-1028/2013)