Effects of Vitamin D Analogs Alfacalcidol and Calcitriol on Cell Proliferation and Migration of HEC1A Endometrial Adenocarcinoma Cells

Abstract

Endometrial carcinoma is the most diagnosed among infiltrating tumor of the female genital tract. Vitamin D has antiproliferative and immunomodulatory properties besides its classical effect on calcium and phosphate. We aimed to demonstrate whether alfacalcidol and calcitriol have any effect on proliferation and migration. Endometrial adenocarcinoma HEC1A was used as a cancer cell line. The effect of alfacalcidol (1α-hydroxyvitamin D₃) and calcitriol (1α,25-dihydroxyvitamin D₃) on proliferation was demonstrated by real-time cell analysis device and migration was shown by a wound healing model. 10⁻¹¹–10⁻⁹M alfacalcidol and calcitriol reduced both proliferation and migration. In contrast, the high concentration of alfacalcidol and calcitriol (10⁻⁸–10⁻⁶M) increased proliferation and migration. The proliferative effects of alfacalcidol (0–12 h) immediately started earlier than calcitriol (12–48 h). In contrast, the antiproliferative effects of calcitriol (12–24 h) have begun earlier than alfacalcidol (48–60 h). While the high concentrations of alfacalcidol and calcitriol increased the migration relatively earlier (12–24 h), low concentrations decreased the migration at late times (24–48 h). Lower concentrations of vitamin D prevent proliferation and migration in endometrial adenocarcinoma HEC1A cells. In contrast, high concentrations of vitamin D increase proliferation and migration.

Disclosure Statement

The authors declare that they have no conflicts of interest.

Additional information

Funding

This study was supported by the Mersin University Scientific Research Projects Center, grant project number: 2015-TP2-1172.