Methanolic Extract of Muntingia Calabura L. Mitigates 1,2-Dimethyl Hydrazine Induced Colon Carcinogenesis in Wistar Rats

Abstract

Objective

The present study aimed to evaluate the efficacy of methanolic extract of Muntingia calabura L. leaves (MEMC) in ameliorating oxidative stress and inflammation associated with 1,2-dimethyl hydrazine (DMH) induced colon cancer.

Methods

The antioxidant enzymes, oxidative stress markers, liver and renal toxicity markers were evaluated. Histopathological examination of colon tissues was carried out with the aid of alcian blue stain and Hematoxylin and Eosin stain.

Results

MEMC supplementation at doses of 100 and 200 mg/kg body weight of rats causes the antioxidant enzymic levels to retain near to its normal range. Meanwhile the oxidative stress markers, which showed an elevation from its normal level upon DMH administration, gets significantly reduced on MEMC treatment. Histopathological observation also revealed that the severity of colorectal cancer was reduced by the supplementation of MEMC.

Conclusion

The findings from the present study showed that MEMC can exert a potential role to ameliorate the oxidative stress and inflammation associated with colorectal cancer.

Authors contributions

Ninan Jisha: work design, performed the experiment, data analysis and Manuscript writing. A. Vysakh: contributed in data processing and manuscript writing. V. Vijeesh: contributed in data analysis. P. S. Anand: assisted in animal experiment. M. S. Latha: work design and result verification.

Acknowledgments

The authors are grateful to School of Biosciences, Mahatma Gandhi University for providing the excellent research facilities. Financial assistance in the form of JRF, to Jisha Ninan from DST-PURSE-PII programme, Government of India, is gratefully acknowledged.

Disclosure statement

No potential conflict of interest was reported by the authors.

Funding

This research work was supported by DST-PURSE-PII (SR/PURSE/Phase2/26(C)) programme, Government of India.