Jab1 Inhibition by Methanolic Extract of Moringa Oleifera Leaves in Cervical Cancer Cells: A Potent Targeted Therapeutic Approach

Abstract

Modern research propounded that alteration in signaling pathways have shown strong connection with the progression of cervical cancer and modulation of these pathways by natural compounds could be a better treatment approach for the management of cervical cancer. Additionally, inhibitory role of methanolic extract of Moringa oleifera leaves against Jab1 has not been elucidated yet. Therefore, we have elucidated the inhibitory potential of MMLE against Jab1 in cervical cancer by analyzing several In Vitro assays. The MMLE treatment in cervical cancer cells showed decreased cell growth in a dose and time dependent manner. Furthermore, MMLE treated cervical cells have also depicted enhanced nuclear condensation (apoptotic induction potential) confirmed by Hoechst analysis. RT-PCR results clearly illustrated the inhibitory potential of MMLE against Jab1 via down regulating its expression. Conversely, a significant increase in p27 expression was also reported which could explain the cells growth arrest at G0/G1 phase. Additionally, enhanced ROS production and caspase-3 activation explains one of the possible mechanisms behind the inhibitory potential of MMLE against Jab1 in cervical cancer cells (HeLa). Thus our experimental findings may pave a potent phyto compound for targeting a crucial signalosome (Jab1) in cervical cancer.

Acknowledgments

We thank Dr. A. P. J. Abdul Kalam Technical University for funding our study and Noida Institute of Engineering and Technology, Greater Noida for its immense support.

Conflict of Interest

I assure that this research study authored by me/us is a genuine and original research work which has neither been submitted for publication elsewhere in any electronic/print.

Additional information

Funding

This study was funded by project grant under “Collaborative research and Innovation Program (CRIP) funding through TEQIP-III of Dr. A. P. J. Abdul Kalam Technical University (AKTU/DEAN-PGSR/2019/CRIP/26)