Abstract
Sulforaphane (SFN), a major isothiocyanate found in cruciferous vegetables, reportedly exerts extensive antitumor effects. Butyl benzyl phthalate (BBP), a widely used plasticizer, plays a crucial role in the promotion of breast cancer. In the present study, we demonstrated that SFN inhibited proliferation, induced apoptosis, and suppressed the stemness of MCF-7 cells, whereas BBP exerted the opposite effects; microRNA-19 (miR-19) plays an important role in BBP-induced cell growth and dysregulation mediated via PTEN and p21. The growth-promoting effect of BBP could be mitigated by SFN, accompanied by a reversal of altered expression of miR-19a, miR-19b, PTEN, and p21. SFN also suppressed BBP-induced binding of upregulated miR-19 with PTEN, as determined using a dual-luciferase reporter assay. Collectively, these results demonstrated, for the first time, that SFN regulates the miR-19/PTEN axis to exert protective effects against BBP-mediated breast cancer promotion, suggesting a new potential role for SFN (or SFN-rich foods) in phthalate antagonism.
Author Contributions
WSC and XML performed experiments and wrote the manuscript. CYZ wrote and edited the manuscript. JSW designed and performed the experiments, and wrote and edited the manuscript.
Conflicts of Interest
All authors declare no potential conflicts of interest relevant to this article.
Ethics Statement
This study did not involve humans or animals, and the cell lines used were purchased from the Chinese Academy of Typical Culture Collection Cell Bank, which conforms to ethical requirements.