Determination of C‐reactive protein in serum and plasma from healthy dogs and dogs with pneumonia by ELISA and slide reversed passive latex agglutination test

Summary

The concentrations of C‐reactive protein (CRP) in serum from dogs diagnosed as normal by clinical, haematological, and biochemical examination were determined by enzyme‐linked immunosorbent assay (ELISA) and slide reversed passive latex agglutination (RPLA), using IgG antibody isolated from rabbit anti‐canine CRP serum. The mean value of CRP in 66 normal dogs kept in private households was 8.4± 4.9 μg/ml by ELISA and 8.5± 6.3 μg/ml by RPLA. Thus, no significant difference was demonstrated between the values obtained by ELISA and RPLA. No significant age and sex‐related differences were found in the CRP values. The mean concentration of CRP in 84 6‐month‐old Beagle dogs kept in kennels by breeders was 6.2±3.9 μg/ml by ELISA and 8.0±4.0 μg/ml by RPLA. Again no significant difference was found. The CRP values determined by ELISA and RPLA were closely correlated (r=0.913). The serum and plasma concentrations of CRP measured by RPLA were also closely correlated (r=0.994). This indicates that plasma can be used as well as serum to determine CRP concentrations by RPLA.

Notes

Immunology and

Veterinary Radiology and

Hygiene, Faculty of Environmental Health. Department of

Veterinary Internal Medicine, School of Veterinary Medicine. Azabu University, Fuchinobe, Sagamihara, Kanagawa 229.

Mie Research Laboratory, Sanwa Kagaku Kenkyusho Co.. Ltd., Hokusei, Inabe.Mie 511–04

Department of Veterinary Science. The National Institute of Health, Shinjuku, Tokyo 162, Japan.