Effect of different artificial aging protocols on marginal sealing ability of self-etch dental adhesives: micro-computed tomography evaluation

Abstract

The aim of this study was to investigate the sealing ability of different self-etch dental adhesives after different aging protocols. Sixty sound human third molars were used for this study. Two standardized Class II box cavities, with the gingival floor 1 mm below the cement-to-enamel junction were prepared in each tooth. Three adhesive groups (Clearfil SE Bond, Clearfil S3 Bond and Single Bond Universal) were subdivided into four subgroups (n = 10) according to the aging protocols: 24 h water storage (control); 1-year water aging; 10,000 thermocycling; and 240,000 thermo-mechanical aging. After the aging protocols, a 50% silver nitrate solution was used as a tracer. The internal adaptation analysis was performed using a micro computed tomography system. All tested aging protocols increase the silver nitrate penetration compared with the controls (p < 0.05). The samples aged in water have no significant difference with the samples aged with thermocycling (p > 0.05). The amount of silver nitrate penetration increased after thermo-mechanical aging for all samples (p < 0.05).The tested adhesives presented different marginal sealing abilities after thermo-mechanical loading and two-step self-etch adhesive showed lower silver nitrate penetration than one-step systems (p < 0.05).

Keywords:

• Self-etch adhesives
• artificial aging
• micro-CT
• sealing ability

Author contributions

Fulya Aydın and Gülbike Demirel contributed to the conception, design, data acquisition, analysis and interpretation, and drafted and critically revised the manuscript. Burak Bilecenoğlu and Mert Ocak contributed to the analysis and data interpretation. Gürkan Gür contributed to the conception and data interpretation and drafted the manuscript. All authors gave final approval and agree to be accountable for all aspects of the work.

Disclosure statement

The authors have no proprietary, financial, or other personal interest of any nature or kind in any product, service, and/or company that is presented in this article.