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Experimental Lung Research Volume 31, 2005 - Issue 3
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DOUBLE-STRANDED RNA STIMULATES THE EXPRESSION OF MONOCYTE CHEMOATTRACTANT PROTEIN-1 IN BEAS-2B BRONCHIAL EPITHELIAL CELLS

Kageaki Taima The Second Department of Internal Medicine, Hirosaki University School of Medicine, Hirosaki, Japan
,
Shingo Takanashi The Second Department of Internal Medicine, Hirosaki University School of Medicine, Hirosaki, Japan
,
Ken Okumura The Second Department of Internal Medicine, Hirosaki University School of Medicine, Hirosaki, Japan
,
Tadaatsu Imaizumi Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine, Hirosaki, Japan
,
Mika Kumagai Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine, Hirosaki, Japan
,
Akira Ishikawa Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine, Hirosaki, Japan
,
Hidemi Yoshida Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine, Hirosaki, Japan
,
Kei Satoh Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine, Hirosaki, Japan
&
Takashi Fujita Department of Tumor Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
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Pages 361-375 | Received 17 Apr 2004, Accepted 25 Nov 2004, Published online: 02 Jul 2009
 
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Abstract

BEAS-2B bronchial epithelial cells were treated with polyinosinic-polycytidylic acid (poly IC), a synthetic double-stranded RNA (dsRNA) analog, and the expressions of monocyte chemoattractant protein-1 (MCP-1) mRNA and protein were analyzed by reverse transcriptase–polymerase chain reaction and enzyme-linked immunosorbent assay. Poly IC enhanced the expression of MCP-1 and release of mononuclear cell chemotactic activity, which were inhibited by dexamethasone pretreatment. The poly IC–induced up-regulation of MCP-1 was blocked by 2-aminopurine, a specific inhibitor of dsRNA-dependent protein kinase, but not by nuclear factor (NF)-κB inhibitor SN50.

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