Abstract
Grapevine grafts (Vitis vinifera L. cv. Riesling, on rootstocks of Vitis sp. L. cv. 5BB) were grown hydroponically in complete nutrient solution (control), Fe‐free nutrient solution and complete nutrient solution with added 10 mM HCO3 −. The concentration of total chlorophyll was significantly reduced in Fe deficient plants, particularly in HCO3 − supplied nutrient solution. The concentration of extracellular (extraplasmatic) root Fe decreased in the case of ‐Fe treatment and increased in that of HCO3 − treatment, while the concentration of symplastic Fe decreased in both treatments. However, the concentration of total root Fe decreased in roots of plants grown in ‐Fe solution and increased in those grown in HCO3 − supplied solution. Fe‐deficient plants showed lowering of total Fe and “active Fe” (extractable in 1 M HCl or o‐phenanthroline) concentration in leaves. These results indicate that bicarbonate in the nutrient solution may be a major inducing factor of Fe‐deficiency chlorosis in grapevine presumably due to inhibited Fe acquisition by roots, but do not confirm Fe inactivation in leaves.