Sulfur Alleviates Growth Inhibition and Oxidative Stress Caused by Cadmium Toxicity in Rice

ABSTRACT

A hydroponic experiment was conducted to investigate the effect of sulfur (S) on growth inhibition and oxidative stress caused by Cd²⁺ toxicity, using two rice cultivars with different grain Cd²⁺ content. Treatments consisted of factorial arrangement of three S levels (0.2, 0.4, and 0.8 mmol), two cadmium (Cd) levels (0 and 1 μ mol), and two rice cultivars (‘Bing 97252,’ a cultivar with low grain Cd²⁺ content, and ‘Xiushui 63,’ a cultivar with high grain Cd²⁺ content). The results showed that Cd²⁺ addition in the medium generally increased Cd²⁺ and malondialdehyde (MDA) content in both roots and shoots; the increases were more pronounced in ‘Xuishui 63’ than in ‘Bing 97252.’ Dramatic reductions in growth parameters, including plant height, root and shoot weight, tillers per plant, chlorophyll content, and net photosynthetic rate were found in the plants exposed to Cd stress relative to the plants without Cd²⁺ treatment. ‘Xiushui 63’ showed more sensitivity than ‘Bing 97252’ under Cd²⁺ exposure. In comparison with the lower S level (0.2 mmol), the higher S levels (0.4 and 0.6 mmol) helped alleviate Cd toxicity, characterized by a significant increase in growth parameters, and a decrease in Cd²⁺ and MDA content in both roots and shoots. In addition, superoxide dismutase (SOD) activity in the plants varied among tissues, cultivars, and Cd treatments. High Cd²⁺ and MDA content was consistently accompanied by higher SOD activity, and higher S levels caused a marked increase in glutathione content and a reduction in SOD activity, indicating a positive effect of S in alleviating oxidative stress.

Keywords:

• cadmium
• growth
• oxidative stress
• rice (Oryza sativa L.)
• sulfur

ACKNOWLEDGMENTS

We are deeply grateful to the Chinese Ministry of Agriculture and Zhejiang Natural Science Foundation for financial support for this research.

Notes

¹Different letters after data within a column represent significant difference at 95% probability.

¹Different letters after data within a column represent significant difference at 95% probability.

¹Different letters after data within a column represent significant difference at 95% probability.

²Only trace was detected.

¹Different letters after data within a column represent significant difference at 95% probability.

^{1 1}Different letters after data within a column represent significant difference at 95% probability.