Inverse Relationship Between Boron Toxicity Tolerance and Boron Contents of Barley Seed and Root

ABSTRACT

A filter-paper bioassay method was used to investigate the differential response of 23 barley (Hordeum vulgare L.) genotypes to boron (B) toxicity. Two-day-old seedlings with equal radicles were treated with 10 (B10) or 100 (B100) ppm B for 10 d. Root and shoot growth was not affected by B10, but root growth was significantly reduced by B100. The shoot growth at B100 was either unaffected or affected to a smaller extent than the root. A significant inverse correlation was found between B content of seed and seed germination, and between root growth and B contents of root and shoot under B100. The barley genotypes with lower B contents in seeds had a higher germination, longer root length, and accumulated less B in roots and shoots when treated with B100. The B-tolerant genotypes with longer roots had lower B contents in their seed, root, and shoot and vice versa. These results suggest that a great variation exists among barley genotypes in response to high B application. There was no difference between naked (uncovered) and covered varieties in response to B100. The B tolerance could be attributed to the lower B content of seed and lower uptake or accumulation of B in the root and shoot.

Keywords:

• Barley genotypes
• boron toxicity
• root
• seed
• shoot

ACKNOWLEDGMENTS

This work was supported by a grant from the BioGreen 21 Program, Rural Development Administration, Republic of Korea.

Notes

∗Significant at P ≤ 0.05;

∗significant at P ≤ 0.01.