Zinc-Induced Changes in Growth Characters, Foliar Properties, and Zn-Accumulation Capacity of Pigeon Pea at Different Stages of Plant Growth

ABSTRACT

Application of zinc (Zn) [50, 100, 200, 300, and 400 μ g zinc sulfate (ZnSO₄)/g of soil] reduced the foliage and the total growth of pigeon pea [Cajanus cajan (Linn.) Huth]. The root-shoot length ratio, varying little with age, was relatively low in the treated plants. Decrease in dry weights of stem and root was more pronounced in the late stages of plant development. The root-shoot dry weight ratio, maximum in the flowering stage, was lower in treated plants than in the control. Number of pods per plant declined, showing a positive correlation with Zn concentration. Net photosynthetic rate, declining with plant age, was significantly low in the treated plants. Density and size of stomata and trichomes, stomatal conductance, intercellular carbon dioxide concentration, quantity of green pigments, nitrate reductase activity, and the nitrate and protein contents in the leaves also declined significantly. Proportion of vascular tissues both in stems and roots increased with plant age with a concomitant reduction of pith and cortex. Under zinc stress, the relative proportion of tissues varied inconsistently. Dimensions of vessel elements and fibers in stems and roots, increasing with the plant age, were always smaller in the treated plants. The vulnerability factor and mesomorphic ratio of treated plants declined, suggesting induction of water stress due to zinc treatments. Accumulation of Zn^{2 +} in different plant parts was considerably high at each developmental stage of the treated plants, and showed a positive correlation with Zn in the soil.

Keywords:

• Bioaccumulation
• biomass
• heavy metal stress
• ontogenetic variation
• photosynthesis
• pigeon pea
• vasculature

ACKNOWLEDGMENTS

We thank Muslim Association for Advancement of Science (MAAS), Aligarh, India for providing a scholarship to the first author, and appreciate Dr. Ehsan A. Khan and Mr. Zakir A. Siddiqui of the Faculty of Science for their help with data analysis, and manuscript preparation respectively.

Notes

**Significant at 1% level

*Significant at 5% level

NS = Non-significant.

**Significant at 1% level

*Significant at 5% level

NS = Non-significant.