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Original Articles

CALIBRATING CHLOROPHYLL METER (SPAD-502) READING BY SPECIFIC LEAF AREA FOR ESTIMATING LEAF NITROGEN CONCENTRATION IN SWEET SORGHUM

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Pages 1640-1646 | Received 10 Mar 2011, Accepted 13 Sep 2011, Published online: 25 Jun 2013
 

Abstract

The chlorophyll meter (SPAD-502) is used to estimate nitrogen status of various crops. However, the relationship between SPAD readings and leaf nitrogen concentration (LNC) in sweet sorghum (Sorghum bicolor) has not been fully established. We examined the relationship between SPAD readings and LNC in sweet sorghum in a two-year study; and the effects of leaf thickness on the relationship was also examined. There was a significant relationship between the SPAD reading and LNC at each of two growth stages, but the correlation was weaker when the data for the two growth stages were pooled. This correlation improved when the specific leaf area was introduced as a second independent variable in the multiple regression analysis. This regression equation was applicable to not only different growth stages but also different seasons. The results suggest that the regression equation developed in this study can help in optimizing nitrogen fertilization for sweet sorghum production.

ACKNOWLEDGMENTS

We thank Mr. K. Papa Rao, Mr. P. V. Satish and Mr. M. Vishwanath, of ICRISAT and Mr. T. Naya of Crop Science Laboratory, Hokkaido University, for their dedicated support. This study was a part of a research project “Development of sustainable soil fertility management for sorghum and sweet sorghum through effective use of biological nitrification inhibition (BNI)” and was funded by grants from the Ministry of Agriculture, Forestry and Fisheries, Japan.

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