Effects of Nitric Oxide on Iron-Deficiency Stress Alleviation of Peanut

Abstract

The aim of this research was to study the role of nitric oxide (NO) in alleviating iron deficiency induced chlorosis of peanut (Arachis hypogaea L.). For this study, sodium nitroprusside (SNP) was used to supply NO for hydroponic peanut plants. After 18 days, the peanut seedlings growing without iron exhibited significant leaf interveinal chlorosis, and this iron-deficiency induced symptom was completely prevented by NO. An increased content of chlorophyll and active iron was observed in NO-treated young leaves, suggesting an improvement of iron availability in plants. In addition, the improved rhizosphere acidification and increased secretion of organic acids by root in NO-treated plants suggesting that NO is effective in modulating iron uptake and transport inside the peanut plants. Furthermore, NO treatment alleviated the increased accumulation of superoxide anion (O₂^•−) and malondialdehyde (MDA), and modulated the antioxidant enzymes. However, the SNP with a prior sunlight treatment that does not release NO had no significant effect on the chlorophyll levels in iron-deficient plants. Therefore, these results support a physiological action of NO on the availability, uptake and transport of iron in the plant.

Keywords:

• Arachis hypogaea L.
• antioxidant enzymes
• iron availability
• organic acids
• rhizosphere acidification

Additional information

Funding

This work was supported by the Projects Shandong Provincial Natural Science Foundation, China (No. ZR2013CM003) and a Project of Shandong Province Higher Educational Science and Technology Program (J14LF08).