Improvement of drought tolerance in Tobacco (Nicotiana rustica L.) plants by Silicon

ABSTRACT

Ameliorative effect of silicon (Si) (2 mM as sodium silicate (Na₂SiO₃)) was studied in tobacco (Nicotiana rustica L.) plants grown under control at 100% field capacity (FC), mild drought (60% FC), and severe drought (30% FC) conditions. Si-treated plants had higher biomass of particularly above-ground parts both under drought and control conditions. Plants with Si supply had significantly higher net assimilation rates but lower transpiration rates. Silicon supply enhanced osmotic potentials only in the leaves, but not in the roots. A considerable rise in the concentrations of soluble sugars was observed particularly in the leaves under both drought and Si treatments. Soluble proteins, free α-amino acids, and proline concentrations increased in Si-treated plants under all watering treatments. Si enhanced the activity of antioxidative enzymes and decreased hydrogen peroxide (H₂O₂) concentrations. Results indicate that Si supplementation alleviates drought stress via improvement of water relation parameters, enhancement of photosynthesis, and elevation of antioxidant defenses.

KEYWORDS:

• antioxidant defense
• organic osmolytes
• photosynthesis
• water use efficiency