Effect of salt stress on tomato plant and the role of calcium

Abstract

Salinity is one of the serious abiotic stresses that has adverse effects on plant growth. The aim of this study was to investigate the effect of sodium chloride (NaCl) on germination and growth parameters of tomato plant as well as the role of Ca²⁺as an ameliorating agent. 100 mM NaCl and two concentrations of calcium (5 and 10 mM) were applied to tomato seeds and seedlings. This study was carried out in a Completely Randomized Design (CRD) with a total of six treatments each comprising of three replicates. The application of 100 mM of NaCl delayed the germination time by 27.6%, reduced the seedling length and seedling vigor by 24.33% and germination stress tolerance by 27.6% as compared to control. Salinity also reduced the plant growth (root and shoot length, root fresh and dry weight, shoot fresh and dry weight, membrane stability, relative water content and leaf area), whereas the application of calcium mitigated the negative effects of salinity on germination and growth to a greater extent. With increased calcium concentration, growth and germination increased significantly both alone and in the salt-affected plant. 10 mM calcium showed best results and enhanced the promptness index by 20.7%, seedling length and vigor by 15.1% and GSI by 20.7%. It also improved root fresh and dry weight, shoot fresh and dry weight, relative water content and leaf area. Similarly, 5 mM calcium also increased plant height and membrane stability index. The present study suggests that application of Ca²⁺ enhanced the growth of tomato plant under saline conditions.

Keywords:

• amelioration
• calcium
• germination and growth
• NaCl
• salt stress
• tomato plant

Acknowledgments

All authors are truly thankful to Mycology Lab, Department of Botany, PMAS AAUR. Without their cooperation, it was impossible to complete our experimental work.

Disclosure statement

No potential conflict of interest was reported by the authors.