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Articles

A novel plant extract as a biostimulant to recover strawberry plants from iron chlorosis

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Pages 2054-2066 | Received 15 Jan 2020, Accepted 30 Mar 2020, Published online: 13 May 2020
 

Abstract

The traditional agricultural production systems are evolving more toward organic, sustainable or environmental friendly systems and products; however, it is important to keep the yield and the quality of crops. The aim of this work was to evaluate the recovery of iron deficient strawberry plants by foliar spray, using a gramineous plants extract (GE). Bare-root transplants of strawberry (Fragaria × ananassa Duch. cv “Diamond”) were grown in a Hoagland’s nutrient solution with iron (Fe10) or without (Fe0). Forty-two days after Fe deprivation, plants grown without iron became chlorotic and GE was applied. Three foliar applications were done, once per week and the regreening was monitored using a SPAD apparatus. After two applications, a rapid but transient regreening was detected in young leaves. Nevertheless, biomass and root/shoot ratio of recovered plants (GE treatment) were similar to chlorotic plants (Fe0 treatment). Phosphorous, Mn and Fe contents were higher in crowns of GE plants at the end of the experiment (67 days). The concentration of organic acids and ferric chelate–reductase (FC-R) activity was also significantly higher in GE treatment. It is possible that the high chelating capacity of the extract was responsible for the Fe internal transport and use.

Disclosure statement

No potential conflict of interest was reported by the authors.

Author contributions

T. Saavedra, P. J. Correia, and M. Pestana wrote the manuscript. T. Saavedra and F. Gama executed the experiments. J. P. Silva and M. G. Miguel performed the LC-MS organic acid determination. A. de Varennes was responsible for mineral composition analysis. All authors contributed to the discussion of the results.

Additional information

Funding

This study was funded by the National Projects (PTDC/AGR-PRO/3861/2012 and UID/Multi/04326/2019) from the Fundação para a Ciência e Tecnologia (FCT) and by the Spanish Ministry of Science and Innovation Project AGL2012–31988 cofinanced by FEDER. T. S, J. P. S., and F. G are thankful to FCT for the Grants SFRH/BD/144764/2019, REEQ/717/QUI/2005, and SFRH/BD/89521/2012, respectively.

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