Ultrastructure of colorectal adenocarcinoma and peritumoral tissue in untreated patients

ABSTRACT

In this study, we describe, compare, and discuss several subcellular alterations found in Colorectal Adenocarcinoma and peritumoral tissue using transmission electron microscopy, morphometry, and statistical analysis. Tissue samples from anterior resections were collected from patients diagnosed with Colorectal Adenocarcinoma in the University Hospital of Caracas. Samples were processed according to the typical protocol for their observation through transmission electron microscopy. The resulting images were analyzed using specialized software for the collection of morphometric data. Several anomalies were common for both tissues, including but not limited to, rough endoplasmic reticulum and mitochondrial swelling, nuclear invagination, nuclear enlargement, and cellular swelling. In general, alterations within the tumor were more frequent and intense. Extensive organellar degradation and other evidences of cellular damage seemed to extend past the edge of the tumor into the peritumoral tissue. There seems to be a clear process of lateral cancerization present in the peritumoral area. The tissue layers composed of smooth muscle cells, probably due to their structural features, may allow greater diffusion of harmful substances produced by the tumor. A more in-depth analysis of peritumoral tissue considering organellar damage and morphometric data may provide relevant insight about the changing microenvironment promoted by the close proximity of a tumor.

KEYWORDS:

• Cancerization
• colorectal
• peritumoral
• tumor
• ultrastructure

Declaration of interest

The authors state that the content of this article is original and has not been published previously nor has been submitted for its publication in another journal. The authors are aware that not disclosing if the submitted manuscript has already been send for publication is considered a severe lack of scientific ethics.

The authors have taken into account the proper ethical guidelines. Through this statement we declare that:

1. The procedures followed during our study conform to the guidelines of the University

2. Hospital of Caracas’s ethics committee, the World Medical Association and the Helsinki Declaration.

3. We guarantee the right to privacy and confidentiality of all patients involved with this study.

4. We have obtained informed consent from all patients involved in this study.

There are no conflicts of interests.