Pollen analysis in some species of Linaceae-Linoideae from Argentina

Abstract

The pollen of Cliococca selaginoides (Lam.) C. M. Rogers & Mildner and eight Linum L. species from Argentina were examined using light and scanning electron microscopy. Both genera share pollen grains which are 3-zonocolpate, isopolar, radiosymmetric, spheroidal and medium to large in size. The sculptural elements of the exine are gemmae or clavae, which allow the genera to be distinguished and the species to be characterised. In Cliococca Bad., the pollen is exclusively gemmate whereas in Linum the pollen is gemmate and clavate (except in L. catharticum L. whose pollen has only gemmae which are apically microechinate). The statistical analyses of quantitative and qualitative morphological characteristics were performed. A key to identify and distinguish the pollen types and sub-types is also provided. A generalized procrustes analysis (GPA) of joint characterisation based on both qualitative and quantitative characters identified four distinct groups.

Keywords:

• Argentina
• Cliococca
• exine
• GPA
• Linum
• sculptural elements

Acknowledgements

We especially thank Eva Acevedo for technical assistance and Brigitte Marazzi for improving an earlier version of this manuscript. We appreciate the attention of Dr James B. Riding and two anonymous reviewers whose detailed comments improved the manuscript. Financial support for our research was provided by the Consejo Nacional de Investigaciones Científicas y Técnicas (PIP N° 112-200801-02248), by the Universidad Nacional del Nordeste (PI A005-2009) and by the Agencia Nacional de Promoción Científica, Tecnológica y de Innovación (ANPCyT-UNNE, PICTO 00096).