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Articles

The use of lithium heteropolytungstate in the heavy liquid separation of samples which are sparse in pollen

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Pages 143-150 | Received 12 Mar 2012, Accepted 01 Oct 2012, Published online: 22 Feb 2013
 

Abstract

We experimented with the use of lithium heteropolytungstate (LST) as a heavy liquid to concentrate pollen in sediments from a desert paleolake with low pollen abundance. LST is one of several non-toxic heavy liquids based on inorganic tungsten that are replacing an older generation of heavy liquids that are harmful to human health, such as zinc bromide, zinc chloride, and bromoform. Several researchers have reported on the use of sodium heteropolytungstate (SPT) in processing pollen samples, but we found no previous reports on using LST in pollen preparation. For our low pollen desert samples, we developed a palynological preparation procedure with LST heavy liquid separation that entails fewer chemical treatments and washes than standard protocols, to minimize laboratory destruction or loss of pollen. In the heavy liquid step, we varied centrifuge times and the specific gravity of the LST solution in an effort to extract the most pollen possible. In the end, our samples proved too pollen-poor to yield countable residues, even under the best circumstances. But our protocols and results can serve as models for experimenting with this new heavy liquid with other sediments.

Acknowledgements

We thank Marith Reheis of the US Geological Survey for providing the Manix core samples, and Matthew Valente and Mathew Boehm for laboratory assistance at the University of Tennessee. We also thank two anonymous reviewers for their comments on this manuscript. Funding for this research was provided by the Friends of Calico. During portions of this research, M. Caffrey was supported by a scholarship from AASP – The Palynological Society and by the National Science Foundation under Grant No. 0550382.

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