ABSTRACT
Melissopalynology, the analysis of pollen grains present in honey, provides relevant information about the pollen and nectar sources in a region utilised by bees for the production of honey, which is used to determine the geographical and botanical origin of the honey. The present investigation is carried out on 20 winter honey samples collected from urban localities of Allahabad to identify the important source plants of the region. The methodology recommended by the International Commission of Bee Botany was followed. Analysis of 20 honey samples recorded a diversity of 62 pollen types, amongst which 49 pollen types were found to originate from entomophilous/amphiphilous taxa, and 13 from anemophilous taxa. Thirteen honey samples were found to be unifloral while the remaining seven samples were multifloral. Brassica campestris, Ageratum conyzoides, Bombax ceiba and Citrus sp. were the predominant pollen types. Fourteen pollen types were recorded in the secondary frequency class, while 27 and 50 pollen types were found in important minor and minor frequency classes, respectively. With regard to the frequency of occurrence of pollen types in honey samples, Brassica campestris, Ageratum conyzoides and Coriandrum sativum were found to be very frequent pollen types as they were recovered from more than 50% of the honey samples. Findings of the present melissopalynological study suggest Brassica campestris, Ageratum conyzoides, Bombax ceiba, Citrus sp. and Coriandrum sativum are the important source plants of Allahabad.
Acknowledgements
Sincere thanks to UGC, New Delhi, India, for providing financial support to the second author under the Rajiv Gandhi National Fellowship, and to Dr M.M. Dwivedi, National Centre of Experimental Mineralogy and Petrology (NCEMP), University of Allahabad, for his help with the scanning electron microscopy. We are also thankful to the reviewers for their useful suggestions.
Disclosure statement
No potential conflict of interest was reported by the authors.
Additional information
Notes on contributors
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Manju Sahney
MANJU SAHNEY, former Head of the Botany Department, has 35 years of teaching experience in botany, University of Allahabad, Allahabad, Uttar Pradesh, India. Her research areas are aerobiology, melissopalynology and plant anatomy.
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Snehlata Rahi
SNEHLATA RAHI is a D Phil student in botany at University of Allahabad. Her research work is on pollen analysis of honey samples.
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Ajay Kumar
AJAY KUMAR is a D Phil student in botany at University of Allahabad. He is about to submit his thesis on melissopalynological investigation on some honeys from Eastern Uttar Pradesh.
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Roma Jaiswal
ROMA JAISWAL is a D Phil student in botany at University of Allahabad, doing research on pollen analysis of spider webs and tree barks.