In Vitro Antimicrobial Activity of Ozonated Sunflower Oil against Antibiotic-Resistant Enterococcus faecalis Isolated from Endodontic Infection

ABSTRACT

The antibacterial efficacy of ozonated sunflower oil against Enterococcus faecalis biofilm was evaluated comparing to gaseous ozone. Sixty disks of composite resin were distributed in sterile microtubes containing bacterial suspension of an antibiotic-resistant E. faecalis isolated from endodontic infection. After incubation for 24 hours at 37°C, six protocols were conducted (n = 10): group I, any treatment was performed; group II, treated with sunflower oil for 10 min; group III, treated with ozonized oil for 5 min; group IV, treated with ozonized oil for 10 min; group V, treated with ozone gas for 2 min; and group VI, treated with ozone gas for 4 min. The control groups I and II showed the highest numbers of colony-forming units (CFU)/mL, with significant differences in comparison to groups treated with ozone-based protocols. There was no significant statistical difference in the number of CFUs between ozone-treated groups. Ozonized oil for 10 min presented the lowest CFU/mL.

KEYWORDS:

• In vitro techniques
• biofilm
• Enterococcus faecalis
• antimicrobial action
• ozone

Declaration of interest statement

The authors declare no conflicts of interest in connection with this article.

Additional information

Funding

This research was financed by UFF/FOPESQ 2017, CAPES, and FAPERJ/JCNE E-26/202.908/2019.