Chemiluminescence from Ozonized Sesame Oil

ABSTRACT

The applications of ozonized oils in human medicine, veterinary, and dentistry have been experiencing a significant increase in the last years. An important chemical property of ozonized oils is their oxidizing capacity, which is directly related to the therapeutical effects. This work aimed to study the light emission caused by the oxidation of luminol by ozonized sesame oil. Sesame oil was ozonized at different time intervals and characterized by peroxide index, Fourier-transform infrared spectroscopy, and thermal analysis. The light emission efficiency was correlated with the pH of the medium, the concentration of horseradish peroxidase (HRP), used as a catalyst, and solvents. A linear correlation was obtained between the concentration of ozonized oil and the integrated light emission. In a second approach, taurine chloramine (Tau-NHCl) was used instead of HRP. The light emission was dependent on both ozonized oil and Tau-NHCl, showing the involvement of intermediate oxidative species in the reaction. Considering the growing therapeutic applications of ozonized oils and Tau-NHCl, these findings instigate further biological investigations to characterize additive or synergic microbicidal properties.

KEYWORDS:

• Chemiluminescence
• luminol
• Ozonized oil
• Sesame oil
• Taurine chloramine

Disclosure statement

No potential conflict of interest was reported by the author(s).

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/01919512.2022.2091511

Additional information

Funding

The authors gratefully acknowledge financial support from National Council for Scientific and Technological Development (CNPq, Conselho Nacional de Desenvolvimento Científico e Tecnológico, grant number: 303485/2019-1), National Institute of Science and Technology - INCT BioNat, grant number: 465637/2014-0, State of Sao Paulo Research Foundation (FAPESP, Fundação de Amparo à Pesquisa do Estado de São Paulo, grant number: 2019/18445-5).