Ozonated Water Promotes in vitro Decontamination of Dental Implants Surface

ABSTRACT

Although studies have shown high survival rates of dental implants, complications may occur mainly due to peri-implant infections. These highlight the importance of establishing protocols for implant decontamination using adjuvant therapies, such as ozone. This study aimed to evaluate the potential for in vitro decontamination of ozonated water on implants surface. Sixteen titanium implants were used, distributed into the groups (n = 4): group E – sterile implants; group C – contaminated implants; group O40 – contaminated implants treated with ozonated water 40 mg/L; and group O60 – contaminated implants treated with ozonated water 60 mg/L. The implants of groups C, O40, and O60 were contaminated in vitro with biofilm. Groups O40 and O60 were decontaminated with ozonated water for 1 minute. Microbiologic analysis was performed by counting colony-forming units (CFUs). Experimental group comparisons were done using a one-way ANOVA test with Tukey’s posttest; a comparison between groups O40 and O60 was done using an independent t-test (p < 0.05). There was a difference among all experimental groups (p = 0.000), with a more significant reduction in CFUs in group O60 (0.83 × 10⁷ ± 0.06) compared to group C (3.41 × 10⁷ ± 0.41). It was concluded that ozonated water 60 mg/L has shown good potential for in vitro decontamination of implants surface.

KEYWORDS:

• Decontamination
• dental implants
• ozone
• peri-implantitis

Disclosure statement

No potential conflict of interest was reported by the authors.