Abstract
A simple and sensitive spectrophotometric method for the determination of hydrogen peroxide, based on the increase in color intensity of dye on addition of surfactant, is described. Hydrogen peroxide is first reacted with potassium iodide in acidic medium, in presence of ammonium molybdate to liberate iodine. The liberated iodine oxidizes leucocrystal violet to crystal violet having maximum absorbance at 593 nm. The color of the dye intensifies with the addition of the surfactant, cetyl pyridinium chloride though the absorption maximum remains the same. Beer's law is obeyed in the range 0.05–0.45 µg of hydrogen peroxide per 25 mL of final solution (0.002–0.018 µg mL−1) with an excellent correlation coefficient (r = 0.9977). The proposed method has been successfully applied for the analysis of hydrogen peroxide in various food products and rainwater.
Notes
Concentration of hydrogen peroxide: 0.01 µg mL−1.
*Tolerance limit may vary the absorbance value by ± 2%.
*Amount: 5 g.
*Mean of three replicate readings.