Study on the Interaction Between Bovine Serum Albumin and Potassium Perfluoro Octane Sulfonate

Abstract

The interactions between potassium perfluorooctanesulfonate (PFOS) and bovine serum albumin (BSA) were studied by fluorescence spectroscopy. The association constants between PFOS and BSA were obtained by fluorescence enhancing and fluorescence quenching respectively. Furthermore, fluorescence quenching was studied at different temperatures, and the binding constant was also determined by the method of fluorescence quenching. According to the thermodynamic parameters, the main binding force could be judged. The experimental results revealed that BSA and PFOS had strong interactions. The mechanism of quenching belonged to dynamic quenching and the main sort of binding force was hydrophobic force. IR-spectra proved the interaction changed the conformation of BSA.

Keywords:

• BSA
• energy transfer
• fluorinated surfactant
• interaction
• quenching

The authors express their deep thanks to the Natural Science Foundation of Hubei Province of China and Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules for supporting this work.

Notes

R is the linear quotiety.