Study on the Binding Equilibrium Between Surfactant FC-134 and DNA by Resonance Light Scattering Technique

Abstract

This article presents the study of binding equilibrium of fluoro surfactant FC-134 to DNA was studied by resonance light scattering. It was found that the interaction resulted in strongly enhanced resonance light scattering signals characterized by the RLS peak at 370 nm. The binding constant Kf and the number of binding n were calculated according to the equilibrium equation, which was established based on the data of the resonance light scattering measurements at 370 nm. It was found that the binding Kf and the number of binding n were 5.98 × 105 and 1.4, respectively.

Keywords:

• Binding equilibrium
• DNA
• fluoro surfactant
• resonance light scattering

Acknowledgments

The Natural Science Foundation of Hubei Province of China and Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules supported the work and all the authors here express their deep thanks.

Notes

0.4 mg · L⁻¹ Cr³⁺; 0.1 mg · L⁻¹ Zn²⁺; 0.5 mg · L⁻¹ Ni²⁺; 0.06 mg · L⁻¹ RNA; 0.05 mg · L⁻¹ Br⁻; 0.2 mg · L⁻¹ Ba²⁺; 1.0 mg · L⁻¹ Ca²⁺.