FRET Between Anthracene and Proflavine Hemisulphate in Micellar Solution and Analytical Application on Determination of Proflavine Hemisulphate

Abstract

Fluorescence resonance energy transfer (FRET) between anthracene and proflavine hemisulphate is studied in micellar solution of sodium dodecyl sulfate. The fluorescence of anthracene is quenched by proflavine hemisulphate (PF) and quenching is accordance with Stern-Volmer relation. The efficiency of energy transfer found to increase with concentration of proflavine hemisulphate. The value of critical energy transfer distance (R₀) calculated by using Foster relation is 19.34 Å and as it is less than 50 Å indicates efficient energy transfer in present system. The optimum conditions for the fluorometric determination of proflavine hemiulphate were studied and the quenching method was successfully applied to estimate the proflavine hemisulphate from the pharmaceutical sample directly.

Keywords:

• Antharcene
• fluorescence quenching
• fluorescence resonance energy transfer (FRET)
• proflavine hemisulphate

Acknowledgments

The authors gratefully acknowledge Department of Science and Technology (DST) and University Grants Commission (UGC), New Delhi for providing Grants to the Department of Chemistry, Shivaji University, Kolhapur under FIST and SAP program, respectively.