Determination of Optimal Reaction Conditions and Influence of Metal Ions on Cellulose Degradation by Cellulase in the Dioctyl Sulfosuccinate Sodium Salt (AOT)/n-Hexanol/Cyclohexane Reversed Micelle System

Abstract

The power-time curves of the cellulose degradation by cellulase were determined under the different conditions with the existence of metal ions or not in the dioctyl sulfosuccinate sodium salt (AOT)/n-hexanol/cyclohexane reverse micelle system by the microcalorimeter method. From these curves, the Michaelis constant and the maximum reaction rate of this reaction were obtained. The optimum T, pH and W_o were determined as W_o = 3.011, pH = 5.015, and T = 315.43 K. Under these optimum reaction conditions, the power-time curves of the cellulose degradation by the cellulase with the metal ions were also obtained. The apparent Michaelis constant and the maximum reaction velocity were gained. The relationship between the apparent Michaelis constant and the concentration of the metal ions was established, and the influence of metal ions was discussed as well. The results indicate that some metal ions have promotion roles on the reaction. However, others could inhibit the reaction. These results are significant for the theoretical and application in the field of reverse micelles enzyme catalysis system.

Keywords:

• Cellulase
• cellulose degradation
• cyclohexane
• dioctyl sulfosuccinate sodium salt (AOT)
• inhibition role
• metal ion
• microcalorimetric method
• n-hexanol
• optimum reaction condition
• promotion role
• reverse micelle