ABSTRACT
Postmenopausal osteoporosis (PMOP) is a chronic bone metabolic disease, which often causes fractures and various complications, it causes a great social and economic burden, and it is urgent to use modern research techniques to elucidate the pathogenesis of PMOP. At the same time, because of the complex physiological and pathological interaction mechanism between osteoporosis and sarcopenia, the correlation research has become a hot topic. Ovary removal is a commonly used experimental method to study the endocrine system of female animals, and it is also the best animal model to study PMOP. In this study, the preparation of the ovariectomized rat was confirmed through the detection of vaginal smear, the level of bone formation markers, and the analysis of bone tissue morphology. Transcriptome sequencing was used to analyze the molecular mechanism of PMOP in ovariectomized rats, qRT-PCR was used to verify the key targets. Results of Micro-CT and scanning electron microscopy (SEM) showed that the trabecular structure was disorganized and the symptoms of osteoporosis appeared, this indicating that the ovariectomized rats model was successfully prepared. Transcriptional sequencing results of femur tissue showed that 452 differentially expressed genes (DEGs) were screened. Bioinformatics analysis results showed that the osteoporosis caused by ovariectomized rats was mainly related to muscle contraction, calcium signaling pathway, etc. Results of qRT-PCR were consistent with transcriptome analysis. These results reveal the pathogenesis of PMOP in ovariectomized rats and also offer a possibility for elucidating the relevance of action between PMOP and sarcopenia.
Disclosure statement
The author(s) declared no potential conflicts of interest with respect to the research, author- ship, and/or publication of this article.
Data availability statement
The transcriptomic data have been deposited to the GEO repository(https://www.ncbi.nlm.nih.gov/geo/info/update.html) with the series record GSE208290.
Ethics statement
This animals experiments was conducted in accordance with relevant regulation of Laboratory Animal Ethics Committee of Henan University of Chinese Medicine (DWLL202003128).
Author contributions
All authors contributed to the article and approved the submitted version.
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Notes on contributors
Shuo Tian
Shuo Tian, Degree: PhD. Affiliation: Academy of Traditional Chinese Medicine, Henan University of Chinese Medicine. Expertise: Chinese medicine, Chinese medicine pharmacology. Detailed-biography (100-200 characters): Mainly devoted to the application of effective components (or effective parts) of traditional Chinese medicine, and explore the mechanism of effect of traditional Chinese medicine.
Yagang Song
Yagang Song, Degree: PhD. Affiliation: Academy of Traditional Chinese Medicine, Henan University of Chinese Medicine. Expertise: Chinese medicine, Chinese medicine pharmacology. Detailed-biography (100-200 characters): Mainly devoted to the application of effective components (or effective parts) of traditional Chinese medicine to explore the mechanism of effect of traditional Chinese medicine.
Jinping Song
Jinping Song, Degree: BD. Affiliation: Academy of Traditional Chinese Medicine, Henan University of Chinese Medicine. Expertise: Chinese medicine, Chinese medicine pharmacology. Detailed-biography (100-200 characters): Mainly devoted to the application of effective components (or effective parts) of traditional Chinese medicine to explore the mechanism of effect of traditional Chinese medicine.
Lin Guo
Lin Guo, Degree: PhD. Affiliation: Department of Pharmacology, Henan University of Chinese Medicine. Expertise: Chinese medicine, Chinese medicine pharmacology. Detailed-biography (100-200 characters): Mainly devoted to the application of effective components (or effective parts) of traditional Chinese medicine to explore the mechanism of effect of traditional Chinese medicine.
Mengfan Peng
Mengfan Peng, Degree: MD. Affiliation: Department of Pharmacology, Henan University of Chinese Medicine. Expertise: Chinese medicine, Chinese medicine pharmacology. Detailed-biography (100-200 characters): Mainly devoted to the application of effective components (or effective parts) of traditional Chinese medicine to explore the mechanism of effect of traditional Chinese medicine.
Xiangxiang Wu
Xiangxiang Wu, Degree: PhD. Affiliation: Academy of Traditional Chinese Medicine, Henan University of Chinese Medicine. Expertise: Chemistry of Chinese materia medica. Detailed-biography (100-200 characters): Mainly devoted to the analysis and identification of active components (or active parts) of traditional Chinese medicine
Jingyi Qiao
Jingyi Qiao, Degree: PhD. Affiliation: Academy of Traditional Chinese Medicine, Henan University of Chinese Medicine. Expertise: Chinese medicine, Chinese medicine pharmacology. Detailed-biography (100-200 characters): Mainly devoted to the application of effective components (or effective parts) of traditional Chinese medicine to explore the mechanism of effect of traditional Chinese medicine.
Ming Bai
Ming Bai, Degree: MD. Affiliation: Academy of Traditional Chinese Medicine, Henan University of Chinese Medicine. Expertise: Chinese medicine, Chinese medicine pharmacology. Detailed-biography (100-200 characters): Mainly devoted to the application of effective components (or effective parts) of traditional Chinese medicine to explore the mechanism of effect of traditional Chinese medicine.
Mingsan Miao
Mingsan Miao, Degree: PhD. Affiliation: Academy of Traditional Chinese Medicine, Henan University of Chinese Medicine. Expertise: Chinese medicine, Chinese medicine pharmacology. Detailed-biography (100-200 characters): Mainly devoted to the application of effective components (or effective parts) of traditional Chinese medicine to explore the mechanism of effect of traditional Chinese medicine.